EA.hy926-CAS9 cell line

EN CN
Scientist.com
Approved Supplier
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > Cas9 Stable Cell Lines > EA.hy926-CAS9
KO Cell Lines
Stable Cell Lines
Wild-type Cell Lines
Stem Cell Lines
Cas9 Stable Cell Lines
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • OVA Stable Cell Lines
  • Stable Cell Lines
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • Wild-type Cell Lines-1 EA.hy926-CAS9-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 EA.hy926-CAS9-Wild-type Cell Lines-2
  • Transfection EA.hy926-CAS9-Transfection
  • EA.hy926-CAS9 cell line EA.hy926-CAS9-产品图
Wild-type Cell Lines-1 EA.hy926-CAS9-Wild-type Cell Lines-1
Wild-type Cell Lines-2 EA.hy926-CAS9-Wild-type Cell Lines-2
Transfection EA.hy926-CAS9-Transfection
EA.hy926-CAS9 cell line EA.hy926-CAS9-产品图
EA.hy926-CAS9 Copy Product Info
Catalog#： YC-C032-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS
STR Report
  • Wild-type Cell Lines-1 EA.hy926-CAS9-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 EA.hy926-CAS9-Wild-type Cell Lines-2
  • Transfection EA.hy926-CAS9-Transfection
  • EA.hy926-CAS9 cell line EA.hy926-CAS9-产品图
EA.hy926-CAS9 Copy Product Info
Catalog#： YC-C032-Cas9-H
Size： 1*10^6
Culture method： 90%DMEM+10%FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Endothelial cells, adherent Culture method 90%DMEM+10%FBS
Passage ratio 1:2~1:3 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Endothelial cells, adherent
Culture method
90%DMEM+10%FBS
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's EA.hy926 cell line was constructed on the basis of wild-type EA.hy926 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in EA.hy926 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

Cutting Efficiency Validation
EA.hy926-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the EA.hy926-CAS9 stable cell pool which is electroporated by SIK1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:EA.hy926		 STR Info (Cell bank)
Cell Line:EA.hy 926
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 8910.08910.0
D16S539 11121112
VWA 14171417
TH01 689.3689.3
AMEL XXXX
TPOX 8989
CSF1PO 101112.0101112.0
D12S391 1518
FGA 2223
D2S1338 2224
D21S11 282932.0
D18S51 131517.0
D8S1179 1313
D3S1358 1516
D6S1043 111222.0
PENTAE 71112.0
D19S433 1314
PENTAD 911
D1S1656 1217
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
Learn More>>>
Learn More>>>

Related Popular Products

KO Cell Lines

  • SIK1 Knockout cell line (EA.hy926)
    Request a Quote More details

    Organism: Human
    Gene Name: SIK1
    Gene ID: 150094
    Size: 1*10^6
    Catalog#: YKO-H1153
    YKO-H1153(EA.hy926)

EGFP Stable Cell Lines

Luciferase Stable Cell Lines

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • SIK1 Knockout cell line (EA.hy926)

    Organism: Human
    Gene Name: SIK1
    Gene ID: 150094
    Size: 1*10^6
    Catalog#: YKO-H1153
    Request a Quote More details

EGFP Stable Cell Lines

Luciferase Stable Cell Lines

Wild-type Cell Lines


WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us
✖

Contact us

If we are unable to reach you via email, how else can we contact you?
✖
Submission Succeeded
Book A Virtual Meeting With Technical Support